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Transposon-mediated BAC transgenesis in zebrafish.

Germline transgenic methods for tracking cells and testing gene function during regeneration in the axolotl. Molecular genetic system for regenerative studies using newts. Generation of transgenic newt Cynops pyrrhogaster for regeneration study. Expressing exogenous genes in newts by transgenesis. I-SceI meganuclease-mediated transgenesis in Xenopus. Pan, F.C., Chen, Y., Loeber, J., Henningfeld, K. I-SceI meganuclease mediates highly efficient transgenesis in fish.

A germline GFP transgenic axolotl and its use to track cell fate: dual origin of the fin mesenchyme during development and the fate of blood cells during regeneration. Sobkow, L., Epperlein, H.H., Herklotz, S., Straube, W.L. The cellular basis for animal regeneration. Regeneration: if they can do it, why can't we? Cell 113, 559–562 (2003). Limb regeneration: a new development? Annu. Fundamental differences in dedifferentiation and stem cell recruitment during skeletal muscle regeneration in two salamander species. In addition, we describe here tamoxifen-induced Cre-mediated recombination in transgenic axolotls. By using Tol2-mediated transgenesis, experiments can be performed within weeks (e.g., 5–6 weeks for obtaining 2–3-cm-long larvae) without the need to establish germline transgenic lines (which take 12–18 months).

Tol2-mediated transgenesis provides different features and advantages compared with I-SceI-mediated transgenesis, and it can result in more than 30% of animals expressing the transgene throughout their bodies so that they can be directly used for experimentation. injection and for axolotl transgenesis using I-SceI meganuclease and the mini Tol2 transposon system. Here we present a protocol for successfully mating and breeding axolotls in the laboratory throughout the year, for metamorphosing axolotls by a single i.p. The axolotl (Mexican salamander, Ambystoma mexicanum) has become a very useful model organism for studying limb and spinal cord regeneration because of its high regenerative capacity.